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血管钠肽抑制异丙肾上腺素增强的大鼠心肌细胞钙瞬变

郭海涛, 朱妙章, 张荣怀, 毕辉, 张博, 张海锋, 于军, 吕顺艳, 裴建明

第四军医大学基础部生理学教研室；第四军医大学西京医院老年病科. 西安 710032

摘要

采用光谱荧光法研究血管钠肽(vasonatrin peptide,VNP)对心肌细胞内钙瞬变的作用及其机制,观察钠尿肽鸟苷酸环化酶(guanylate cyclase,GC)受体的特异性阻断剂(HS-142-1)、8-溴-环磷酸鸟苷(8-Br-cGMP)和镁蓝(methylene blue,MB)对心肌细胞内钙瞬变的影响.结果显示,异丙肾上腺素(isoproterenol,Iso)(10-10～10+-6mol/L)可剂量依赖性地引起心肌细胞内钙瞬变增强。

关键词： 血管钠肽; 心肌细胞; cGMP; 镁蓝; 钙瞬变

Vasonatrin peptide attenuates the enhancement of electrically-induced intracellular calcium transient by isoproterenol in rat cardiac myocytes

Guo Haitao, Zhu Miaozhang, Zhang, Ronghuai, Bi Hui, Zhang Bo, Zhang Haifeng, Yu Jun, Lu Shunyan

Department of Physioloy and, Department of Geriatrics of Xijing Hospital, Fourth Military Medical University. Xi'an 710032, China

Abstract

The purpose of this study was to investigate the effects of vasonatrin peptide (VNP) on electrically-induced intracellular calcium [Ca~(2+)] transient and mechanism of the effect in the cardiac myocytes. The [Ca~(2+)] transient was measured with a fluoremetric method. The effects of HS-142-1,8-Br-cGMP and methylene blue (MB) on [Ca~(2+)] transient in cardiac myocytes were also determined. Isoproterenol (Iso) at 10~(-10)-10~(-6) mol/L augmented electrically-induced [Ca~(2+)], transient dose-dependently.

Key words: Vasonatrin peptide;Cardiac myocytes;Cyclic GMP;Methylene blue;Intracellular Calcium transient

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引用本文：

郭海涛, 朱妙章, 张荣怀, 毕辉, 张博, 张海锋, 于军, 吕顺艳, 裴建明. 血管钠肽抑制异丙肾上腺素增强的大鼠心肌细胞钙瞬变[J]. 生理学报 2004; 56 (3): .

Guo Haitao, Zhu Miaozhang, Zhang, Ronghuai, Bi Hui, Zhang Bo, Zhang Haifeng, Yu Jun, Lu Shunyan. Vasonatrin peptide attenuates the enhancement of electrically-induced intracellular calcium transient by isoproterenol in rat cardiac myocytes. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).